The plumbing is all new and the display tank has been segregated from the sump and the frag tank. The frag tank, sump, and all associated plumbing has had a vinegar water mixture running through it for a few days. The ph was holding steady at abou 5.5 or so and I'm fairly certain that all living things have been killed off. To include the copepods and amphipods unfortunately. I've since drained the tanks, rinsed them, drained them again and filled with fresh RO water and salt. Later on tonight I will be turning on the main pump and will start the siphon on the DT. Here we go!