Yes tank I’m treating is actually my main tank. I removed all corals and inverts a few months back when dosed cupramine for first time. Tank is 240g with 125g sump 300g of water. Test kit I’ve been using is Seachem. Reason I’m treating in main tank is because I don’t have a big enough qt tank for all my fish as I have 4 bigger tangs alone and 10 fish total. At this point I don’t care if I can hold corals in the tank again as more worried about my fish as have few higher end or harder to find fish. I have a small coral tank if need for that. As far as carbon or other media filters I have removed all carbon and cuprisorb I was running from treatment few months ago as need to treat again. Skimmer and uv are both off. Only thing I have besides powerhead and heater running is an air pump to help with depleted O2 and filter socks.