Yes, I am using API for testing, And I like your idea of testing on fresh salt water as a control. I think I'll dose some bacteria in the tank to help reduce the ammonia level and reduce stress on the fish. I wish now I had used another method other than introducing a fish to get the cycling started, but that was suggested to me at the time I was getting what I needed to set up the tank. By the way, is there a way by observing the fish to tell that he is being stressed?